Screening for mutations in human alpha-globin genes by nonradioactive single-strand conformation polymorphism.

DNA Polymorphisms at Candidate Gene Loci and Their Relation with Milk Production Traits in Murrah Buffalo (Bubalus bubalis)

DNA polymorphism within diacylglycerol transferase 2 (DGAT2) / monoacyl glycerol transferases 2 (MOGAT2), leptin and butyrophilin genes were analysed using PCR-SSCP in Murrah buffalo. The single strand conformation polymorphism (SSCP) analysis of amplified gene fragment in exon 5 of MOGAT2, exon 3 of leptin and intron 1 of butyrophilin gene revealed different patterns. A, B and C showed the fol...

Plastic versus glass tubes: effects on analytical performance of selected serum and plasma hormone assays.

DGGE and SSCA. Hum Mutat 1996;7:114–22. 6. Jorge SB, Melo MB, Costa FF, Sonati MF. Screening for mutations in human -globin genes by nonradioactive single-strand conformation polymorphism. Braz J Med Biol Res 2003;36:1471–4. 7. Foglietta E, Deidda G, Graziani B, Modiano G, Bianco I. Detection of -globin gene disorders by a simple PCR methodology [Erratum published in: Haematologica 1996;81:XVI]...

Carrier Determination in a Hemophilia B Family Using Single Strand Conformation Polymorphism (SSCP) and Sequencing

Single-strand conformation polymorphism analysis to detect p53 mutations: characterization and development of controls.

Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified p53 exons 4-8, where mutations frequently occur in human tumors. Easily obtain...

تعیین جهش در اگزون 8 ژن p53 در بیماران مبتلا به تومور مغزی از نوع آستروسایتوما

Background: Most studies have shown that there are association between the development and malignancy of brain tumors and tumor suppressor genes and oncogenes. The aim of this project was to investigate the P53 gene mutations in exon 8 in patients with astrocytoma type’s brain tumor. Methods: In this present survey, The DNA isolation from 30 samples of brain tissue was done by phenol-c...